Quantitation of mRNA by Polymerase Chain Reaction :Nonradioactive PCR Methods - Springer Lab Manuals
Quantitation of mRNA by Polymerase Chain Reaction :Nonradioactive PCR Methods - Springer Lab Manuals
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Published:
29 December, 2011
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Published:
29 December, 2011
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Description
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
More Details
| Type | Book |
|---|---|
| ISBN13 | 9783642797149 |
| ISBN10 | 3642797148 |
| Number Of Pages | 166 |
| Item Weight | 1000 g |
| Publisher / Reseller | Springer-Verlag Berlin and Heidelberg GmbH & Co. KG |
| Format | paperback |
| Edition | Softcover reprint of the original 1st ed. 1995 |
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Media Reviews
"...this technical book will be very useful for all customers of PCR. And they are numerous!... Summarizing: an excellent booklet, which we recommend highly and absolutely necessary to all practicians of PCR products and their applications." Cellular and Molecular Biology