Maintaining consistent and reliably high success rates is a daily challenge for every IVF laboratory. This step-by-step guide is an essential aid in navigating the complex maze of physical, chemical, biological, and logistic parameters that underpin successful gamete and embryo culture: temperature, pH, osmolality, gas supplies, air quality, light exposure, infections, managing supplies, personnel, as well as overall quality control. Numerous real-life troubleshooting case reports are presented, identifying all aspects necessary for troubleshooting. Process maps and flow charts accompanying each chapter offer a logical and systematic approach to problem solving in the laboratory. This is an essential resource for scientists in assisted reproductive technology and specialists in reproductive biology and medicine, helping IVF clinics to achieve the dream of every infertile couple: the birth of a healthy child.

'This book is an essential read for embryologists at all stages of their careers … This book will prompt reflection on current laboratory practice and has sparked lively and thought-provoking discussions with my colleagues. No embryologist should be without it!' Lucy Richardson, The Bulletin

Kay Elder is a Senior Research Scientist at Bourn Hall Clinic, Bourn, Cambridge, UK. Marc Van den Bergh is Chief Executive Officer, Quartec GmbH, Fislisbach, Switzerland. Bryan Woodward is a freelance Consultant Clinical Embryologist based in Leicestershire, UK.